Difference between revisions of "Part:BBa K2721000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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To express BioBricks under the control of this promoter, similar to the wild-type T7 promoter, the E.coli chassis should carry a T7 polymerase gene.
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This promoter is quite short. So we recommend PCR to assembly it with protein coding sequence.
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===Source===
 
===Source===

Revision as of 12:12, 1 September 2018


Modified T7 promoter 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter is quite short. So we recommend PCR to assembly it with protein coding sequence.

Source

BBa_R0085

References