Difference between revisions of "Part:BBa K2810008:Design"

(Created page with " __NOTOC__ <partinfo>BBa_K2810008 short</partinfo> <partinfo>BBa_K2810008 SequenceAndFeatures</partinfo> ===Design Notes=== Cloned into golden gate compatible pSB1C3 ===...")
 
 
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===Design Notes===
 
===Design Notes===
−
Cloned into golden gate compatible pSB1C3
+
The part is GoldenGate compatible and so has 5' and 3' sticky ends of 4bases to enable the part to ligate into pGBA2. It also has short 3 nucleotide sequences at each end, that when ligated into the pGBA2 plasmid, creates a recognition site for the Type II S restriction enzyme, BsaI.
  
  
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===Source===
 
===Source===
  
−
The aphid Myzus persisae
+
Myzus persicae genomic sequence
  
 
===References===
 
===References===

Latest revision as of 11:01, 31 August 2018


siRNA* from 5' region of Myzus persicae SP3 secreted protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is GoldenGate compatible and so has 5' and 3' sticky ends of 4bases to enable the part to ligate into pGBA2. It also has short 3 nucleotide sequences at each end, that when ligated into the pGBA2 plasmid, creates a recognition site for the Type II S restriction enzyme, BsaI.


Source

Myzus persicae genomic sequence

References