Difference between revisions of "Part:pSB1A7"

 
 
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<partinfo>BBa_J31009 short</partinfo>
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<partinfo>pSB1A7 short</partinfo>
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pSB1A7 was constructed by adding double terminators ([https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015]) before the BioBrick prefix and after the BioBrick suffix in [https://parts.igem.org/cgi/partsdb/part_info.cgi?part_name=pSB1A3 pSB1A3]. The added terminators block read-through transcription coming from the vector backbone into the BioBrick MCS.
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pSB1A7 was promoted from BBa_J31009 by Randy on 11/16/2006.
  
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pSB1A4 was constructed by adding double terminators ([BBa_B0015]) before the BioBrick prefix and after the BioBrick suffix in pSB1A3. This insulator blocks read-through transcription into BioBrick parts/ devices.
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pSB1A8 is a newer, modified version of pSB1A7 with the BsaI site in the ampR gene removed [https://parts.igem.org/Part:BBa_J119043 (J119043)].
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_J31009 SequenceAndFeatures</partinfo>
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<partinfo>pSB1A7 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_J31009 parameters</partinfo>
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<partinfo>pSB1A7 parameters</partinfo>
 
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Latest revision as of 17:32, 12 July 2018

Transcriptionally insulated high copy BioBrick plasmid

pSB1A7 was constructed by adding double terminators (BBa_B0015) before the BioBrick prefix and after the BioBrick suffix in pSB1A3. The added terminators block read-through transcription coming from the vector backbone into the BioBrick MCS.

pSB1A7 was promoted from BBa_J31009 by Randy on 11/16/2006.

pSB1A8 is a newer, modified version of pSB1A7 with the BsaI site in the ampR gene removed (J119043).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2411
    Illegal XbaI site found at 2426
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2411
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal NotI site found at 8
    Illegal NotI site found at 2417
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2411
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2411
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2426
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1312