Difference between revisions of "Part:BBa J119043"
 
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<partinfo>BBa_J119043 short</partinfo>
 
<partinfo>BBa_J119043 short</partinfo>
  
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Insulated vector based on pSB1A2.  Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix. There is also a spontaneous mutation in the Ori such that the copy number is much lower than expected. This feature can be advantageous if you don't want a toxic protein to be expressed in your host cells.  
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Insulated vector based on pSB1A2.  Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix. The Bsa I site was removed from the ampicillin resistance gene in pSB1A7 [https://parts.igem.org/Part:pSB1A7 (pSB1A7)]. There is also a spontaneous mutation in the Ori such that the copy number is much lower than expected. This feature can be advantageous if you don't want a toxic protein to be expressed in your host cells.  
  
 
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Latest revision as of 17:29, 12 July 2018


pSB1A8

Insulated vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix. The Bsa I site was removed from the ampicillin resistance gene in pSB1A7 (pSB1A7). There is also a spontaneous mutation in the Ori such that the copy number is much lower than expected. This feature can be advantageous if you don't want a toxic protein to be expressed in your host cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2207
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2207
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2207
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2207
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2207
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2222
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.