Difference between revisions of "Part:BBa K2571000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
The enzyme L-1,2-propanediol oxidoreductase (encoded by FucO) is an NADH-linked, iron-dependent group III dehydrogenase. Expression of fucO from plasmids has been used to improve furfural tolerance in Escherichia coli-based fermentations for ethanol and lactic acid. The reduction of furfural to less toxic alcohol seems essential for growth and fermentation of dilute acid hydrolysates of hemicellulose. | The enzyme L-1,2-propanediol oxidoreductase (encoded by FucO) is an NADH-linked, iron-dependent group III dehydrogenase. Expression of fucO from plasmids has been used to improve furfural tolerance in Escherichia coli-based fermentations for ethanol and lactic acid. The reduction of furfural to less toxic alcohol seems essential for growth and fermentation of dilute acid hydrolysates of hemicellulose. | ||
Revision as of 12:52, 9 July 2018
FucO/ L-1,2-Propanediol Oxidoreductase
FucO: 1,2-propanediol oxidoreductase from E. coli has NADH-dependent furan reductase activity. L-1,2-propanediol oxidoreductase is an iron-dependent group III dehydrogenase
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Zheng, H., Wang X., Yomano, L.P., Geddes R.Y., Shanmugam, K.T., Ingram, L.O. (2013). Improving Eschericia coli FucO for Furfural Tolerance by Saturation Mutagenesis of Individual Amino Acid Positions. Applied and Environmental Microbiology. May 2013, Volume 79, Number 10. p. 3202-3208.