Difference between revisions of "Part:BBa K2571000"
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===Usage and Biology===
 
===Usage and Biology===
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The enzyme L-1,2-propanediol oxireductase (encoded by FucO) is an NADH-linked, iron-dependent group III dehydrogenase. Expression of fucO from plasmids has been used to improve furfural tolerance in Escherichia coli-based fermentations for ethanol and lactic acid. The reduction of furfural to less toxic alcohol seems essential for growth and fermentation of dilute acid hydrolysates of hemicellulose.
  
 
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<partinfo>BBa_K2571000 parameters</partinfo>
 
<partinfo>BBa_K2571000 parameters</partinfo>
 
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===References===
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Zheng, H., Wang X., Yomano, L.P., Geddes R.Y., Shanmugam, K.T., Ingram, L.O. (2013). Improving Eschericia coli FucO for Furfural Tolerance by Saturation Mutagenesis of Individual Amino Acid Positions. Applied and Environmental Microbiology. May 2013, Volume 79, Number 10. p. 3202-3208.

Revision as of 12:36, 9 July 2018


FucO/ L-1,2-Propanediol Oxidoreductase

FucO: 1,2-propanediol oxidoreductase from E. coli has NADH-dependent furan reductase activity. L-1,2-propanediol oxidoreductase is an iron-dependent group III dehydrogenase

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Zheng, H., Wang X., Yomano, L.P., Geddes R.Y., Shanmugam, K.T., Ingram, L.O. (2013). Improving Eschericia coli FucO for Furfural Tolerance by Saturation Mutagenesis of Individual Amino Acid Positions. Applied and Environmental Microbiology. May 2013, Volume 79, Number 10. p. 3202-3208.