Difference between revisions of "Part:BBa J100416"
 
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<partinfo>BBa_J100416 short</partinfo>
 
<partinfo>BBa_J100416 short</partinfo>
  
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T7 consensus sequence <a href="https://parts.igem.org/Part:BBa_R0085">(R0085)</a> inserted into pClone Red <a href="https://parts.igem.org/Part:BBa_J119137">(J119137)</a>, encoding for RFP. Used as a control to test against experimental promoters that had Gal4 binding sites upstream, downstream, and within this promoter sequence.  
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T7 consensus sequence [https://parts.igem.org/Part:BBa_R0085 (R0085)] inserted into pClone Red [https://parts.igem.org/Part:BBa_J119137 (J119137)], encoding for RFP. Used as a control to test against experimental promoters that had Gal4 binding sites upstream, downstream, and within this promoter sequence.  
  
 
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Latest revision as of 14:10, 26 June 2018


T7 Consensus Promoter in pClone Red

T7 consensus sequence (R0085) inserted into pClone Red (J119137), encoding for RFP. Used as a control to test against experimental promoters that had Gal4 binding sites upstream, downstream, and within this promoter sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1437
    Illegal AgeI site found at 1549
  • 1000
    COMPATIBLE WITH RFC[1000]