Difference between revisions of "Part:BBa M50435:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The entC | + | The entC nucleotide sequence was obtained by codon-optimizing (for ''E. coli'') the amino acid sequence obtained from UniProt, using IDT's reverse translation tool. A 6xHis tag was appended to the C terminus for antibody detection and assay purposes (Western Blot, ELISA, etc.) |
===Source=== | ===Source=== | ||
| − | + | Organism: ''E. coli'' <BR> | |
| − | https://www.uniprot.org/uniprot/P0AEJ2 | + | UniProt amino acid sequence: https://www.uniprot.org/uniprot/P0AEJ2 <BR> |
| + | 6xHis: BBa_K133133 | ||
===References=== | ===References=== | ||
| + | https://www.uniprot.org/uniprot/P0AEJ2 <BR> | ||
| + | https://parts.igem.org/Part:BBa_K133133 <BR> | ||
| + | Ma, L. & Payne, S. “AhpC is Required for Optimal Production of Enterobactin by Escherichia coli”. Journal of Bacteriology, 2012. http://jb.asm.org/content/194/24/6748.long | ||
Latest revision as of 06:08, 12 June 2018
entC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The entC nucleotide sequence was obtained by codon-optimizing (for E. coli) the amino acid sequence obtained from UniProt, using IDT's reverse translation tool. A 6xHis tag was appended to the C terminus for antibody detection and assay purposes (Western Blot, ELISA, etc.)
Source
Organism: E. coli
UniProt amino acid sequence: https://www.uniprot.org/uniprot/P0AEJ2
6xHis: BBa_K133133
References
https://www.uniprot.org/uniprot/P0AEJ2
https://parts.igem.org/Part:BBa_K133133
Ma, L. & Payne, S. “AhpC is Required for Optimal Production of Enterobactin by Escherichia coli”. Journal of Bacteriology, 2012. http://jb.asm.org/content/194/24/6748.long