Difference between revisions of "Part:BBa M50436:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | We acquired the base pair sequence for this protein by running the | + | We acquired the base pair sequence for this protein by running the UniProt amino acid sequence for ahpC through the Integrated DNA Technologies (IDT) Codon Optimization software, optimized for ''E. coli''. We then added a FLAG sequence at the C-terminus so that our protein could be easily visualized using a Western Blot. |
===Source=== | ===Source=== | ||
+ | Organism: ''E. coli'' <BR> | ||
− | Uniprot Amino Acid Sequence: https://www.uniprot.org/uniprot/P0AE08 | + | Uniprot Amino Acid Sequence: https://www.uniprot.org/uniprot/P0AE08 <BR> |
− | + | ||
− | + | ||
+ | FLAG sequence: BBa_T2004 | ||
===References=== | ===References=== |
Latest revision as of 06:08, 12 June 2018
ahpC
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 58
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We acquired the base pair sequence for this protein by running the UniProt amino acid sequence for ahpC through the Integrated DNA Technologies (IDT) Codon Optimization software, optimized for E. coli. We then added a FLAG sequence at the C-terminus so that our protein could be easily visualized using a Western Blot.
Source
Organism: E. coli
Uniprot Amino Acid Sequence: https://www.uniprot.org/uniprot/P0AE08
FLAG sequence: BBa_T2004
References
https://www.uniprot.org/uniprot/P0AE08
https://parts.igem.org/Part:BBa_T2004
Ma, L. & Payne, S. “AhpC is Required for Optimal Production of Enterobactin by Escherichia coli”. Journal of Bacteriology, 2012. http://jb.asm.org/content/194/24/6748.long