Difference between revisions of "Part:BBa M50435:Design"
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===Design Notes===
 
===Design Notes===
−
The entC amino acid sequence obtained from UniProt was codon optimized for ''E. coli'' using IDT's reverse translation tool. A 6xHis tag was appended to the C terminus for antibody detection and assay purposes (Western Blot, ELISA, etc.)
+
The entC nucleotide sequence was obtained by codon-optimizing (for ''E. coli'') the amino acid sequence obtained from UniProt, using IDT's reverse translation tool. A 6xHis tag was appended to the C terminus for antibody detection and assay purposes (Western Blot, ELISA, etc.)
  
  

Revision as of 06:00, 12 June 2018


entC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The entC nucleotide sequence was obtained by codon-optimizing (for E. coli) the amino acid sequence obtained from UniProt, using IDT's reverse translation tool. A 6xHis tag was appended to the C terminus for antibody detection and assay purposes (Western Blot, ELISA, etc.)


Source

UniProt amino acid sequence: https://www.uniprot.org/uniprot/P0AEJ2 6xHis: BBa_K133133

References

https://www.uniprot.org/uniprot/P0AEJ2
https://parts.igem.org/Part:BBa_K133133
Ma, L. & Payne, S. “AhpC is Required for Optimal Production of Enterobactin by Escherichia coli”. Journal of Bacteriology, 2012. http://jb.asm.org/content/194/24/6748.long