Difference between revisions of "Part:BBa M50437:Design"
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Our plasmid contained two distinct coding sequences, separately delineated with ribosome-binding sites. | Our plasmid contained two distinct coding sequences, separately delineated with ribosome-binding sites. | ||
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| + | The coding sequences were obtained from reverse translation and optimization of UniProt amino acid sequences, and other parts were obtained from the iGEM parts registry. | ||
===Source=== | ===Source=== | ||
Revision as of 23:47, 11 June 2018
2,3-DHB Biosynthesis Construct, with entC and ahpC
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 234
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 234
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 234
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 234
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 234
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Our plasmid contained two distinct coding sequences, separately delineated with ribosome-binding sites.
The coding sequences were obtained from reverse translation and optimization of UniProt amino acid sequences, and other parts were obtained from the iGEM parts registry.
Source
T5 promoter: BBa_M50075
Strong RBS: BBa_M50080
entC: BBa_M50436
ahpC: BBa_M50435
T7 terminator: BBa_M50080