Difference between revisions of "Part:BBa M50440:Experience"
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===Applications of BBa_M50440=== | ===Applications of BBa_M50440=== | ||
| + | This construct can successfully be used to measure intracellular pH given by a BRET ratio in S. cerevisiae. Due to the well-characterized pH-sensitivity of Rluc8 (luciferase), we are confident that BRET readout from cpVenus (fluorophore) is indicative of intracellular pH because it was excited through Rluc8 activity. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 17:40, 11 June 2018
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Applications of BBa_M50440
This construct can successfully be used to measure intracellular pH given by a BRET ratio in S. cerevisiae. Due to the well-characterized pH-sensitivity of Rluc8 (luciferase), we are confident that BRET readout from cpVenus (fluorophore) is indicative of intracellular pH because it was excited through Rluc8 activity.
User Reviews
UNIQbc2b74976a9c3acc-partinfo-00000000-QINU UNIQbc2b74976a9c3acc-partinfo-00000001-QINU