Difference between revisions of "Part:BBa J100400"

 
 
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<partinfo>BBa_J100400 short</partinfo>
 
<partinfo>BBa_J100400 short</partinfo>
  
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Started with pClone mScarlet (J100397) and performed golden gate assembly (GGA) on it using oligos for the promoter J100371  from the PM "blue" bio113 spring 2018 group.  
+
Started with pClone mScarlet ([https://parts.igem.org/Part:BBa_J100397 J100397]) and performed golden gate assembly (GGA) on it using oligos for the promoter [https://parts.igem.org/Part:BBa_J100371 J100371] from the PM "blue" Bio113 spring 2018 group.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:52, 21 May 2018


pClone mScarlet + promoter "blue"

Started with pClone mScarlet (J100397) and performed golden gate assembly (GGA) on it using oligos for the promoter J100371 from the PM "blue" Bio113 spring 2018 group.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 917
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 917
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 917
    Illegal BamHI site found at 863
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 917
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 917
  • 1000
    COMPATIBLE WITH RFC[1000]