Difference between revisions of "Part:BBa I718008:Experience"
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A strain bearing the cassette lox-kanR-lox inserted into its chromosome was transformed with BBa_I718008 in the pSB1A2 plasmid. Directly after transformation, liquid cultures were launched with either Amp or Amp+Kan. 100µl of the cultures were regularly plated at appropriated concentrations on LB+Amp, giving the following growth curves: | A strain bearing the cassette lox-kanR-lox inserted into its chromosome was transformed with BBa_I718008 in the pSB1A2 plasmid. Directly after transformation, liquid cultures were launched with either Amp or Amp+Kan. 100µl of the cultures were regularly plated at appropriated concentrations on LB+Amp, giving the following growth curves: | ||
| − | [[Image: Paris_RecombinationCurve. | + | [[Image: Paris_RecombinationCurve.jpg|center|600px]] |
[[Image:Paris_RecombinationRate.JPG|center]] | [[Image:Paris_RecombinationRate.JPG|center]] | ||
Revision as of 00:02, 27 October 2007
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I718008
This Cre recombinase generator can be used to induce recombination between two lox site at a tunable rate. An experience conducted by iGEM Paris 2007 gives the recombination rate without induction:
A strain bearing the cassette lox-kanR-lox inserted into its chromosome was transformed with BBa_I718008 in the pSB1A2 plasmid. Directly after transformation, liquid cultures were launched with either Amp or Amp+Kan. 100µl of the cultures were regularly plated at appropriated concentrations on LB+Amp, giving the following growth curves:
User Reviews
UNIQ07dde48aa314d6c3-partinfo-00000000-QINU UNIQ07dde48aa314d6c3-partinfo-00000001-QINU