Difference between revisions of "Part:BBa I718008:Experience"

Revision as of 00:01, 27 October 2007

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Applications of BBa_I718008

This Cre recombinase generator can be used to induce recombination between two lox site at a tunable rate. An experience conducted by iGEM Paris 2007 gives the recombination rate without induction:

A strain bearing the cassette lox-kanR-lox inserted into its chromosome was transformed with BBa_I718008 in the pSB1A2 plasmid. Directly after transformation, liquid cultures were launched with either Amp or Amp+Kan. 100µl of the cultures were regularly plated at appropriated concentrations on LB+Amp, giving the following growth curves:

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User Reviews

UNIQ3f8e33589a26ddfc-partinfo-00000000-QINU UNIQ3f8e33589a26ddfc-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_I718008===
+This Cre recombinase generator can be used to induce recombination between two lox site at a tunable rate. An experience conducted by iGEM Paris 2007 gives the recombination rate without induction:
+A strain bearing the cassette lox-kanR-lox inserted into its chromosome was transformed with BBa_I718008 in the pSB1A2 plasmid. Directly after transformation, liquid cultures were launched with either Amp or Amp+Kan. 100µl of the cultures were regularly plated at appropriated concentrations on LB+Amp, giving the following growth curves:
+[[Image: Paris_RecombinationCurve.png|center|600px]]
+[[Image:Paris_RecombinationRate.JPG|center]]
 ===User Reviews===