Difference between revisions of "Part:BBa J100397:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
  
 
===Applications of BBa_J100397===
 
===Applications of BBa_J100397===
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Measure of relative Fluorescence.
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The red bars are pClone RFP samples, and the pink bars are mScarlet samples. Experiment 3 has two RFP bars because the DNA was taken from two separate freezer stocks. Error bars are standard deviation from 3 reads of the same overnight culture. RFP was measured using 585 nm (ex) and 615 nm (em). mScarlet was measured with 569 nm (ex) and 594 nm (em). Absorbance was 600 nm. Relative fluorescence was calculated by dividing fluorescence by absorbance values.
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[[File:J100397.png|500px|]]
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===User Reviews===
 
===User Reviews===

Latest revision as of 12:33, 18 May 2018


Applications of BBa_J100397

Measure of relative Fluorescence. The red bars are pClone RFP samples, and the pink bars are mScarlet samples. Experiment 3 has two RFP bars because the DNA was taken from two separate freezer stocks. Error bars are standard deviation from 3 reads of the same overnight culture. RFP was measured using 585 nm (ex) and 615 nm (em). mScarlet was measured with 569 nm (ex) and 594 nm (em). Absorbance was 600 nm. Relative fluorescence was calculated by dividing fluorescence by absorbance values.

J100397.png

User Reviews

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