Difference between revisions of "Part:BBa I741001:Experience"
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The HSL Receiver construct restores motility in motB knockout strain RP3087 on induction with HSL.  The plasmid is sensitive to HSL concentrations as low as 1nM (publication pending).  Assays have been completed by mixing cells producing AHL with "Receiver Cells" and plating on low concentrations of agar (~.3%).  Confirmation of AHL production can be achieved by visualization of swarming (shown below).
 
The HSL Receiver construct restores motility in motB knockout strain RP3087 on induction with HSL.  The plasmid is sensitive to HSL concentrations as low as 1nM (publication pending).  Assays have been completed by mixing cells producing AHL with "Receiver Cells" and plating on low concentrations of agar (~.3%).  Confirmation of AHL production can be achieved by visualization of swarming (shown below).
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[[Image:Swarming_cells_induced_with_AHL.JPG|thumb|left|Swarm Radius]]
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===User Reviews===
 
===User Reviews===
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<I>Username</I>
 
<I>Username</I>
 
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I have been working with this part for several months, in which it has consistantly worked.  Data will be uploaded soon.
 
 
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I have been working with this part for several months, in which it has consistently worked.  Leaky expression does appear to be a problem after 24+ hours, but it is quite stable until then.
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note: the receiver construct is currently hosted on a psb2K3 plasmid.

Latest revision as of 23:58, 26 October 2007

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I741001

The HSL Receiver construct restores motility in motB knockout strain RP3087 on induction with HSL. The plasmid is sensitive to HSL concentrations as low as 1nM (publication pending). Assays have been completed by mixing cells producing AHL with "Receiver Cells" and plating on low concentrations of agar (~.3%). Confirmation of AHL production can be achieved by visualization of swarming (shown below).

Swarm Radius




User Reviews

UNIQ96a036b06d249cf2-partinfo-00000000-QINU UNIQ96a036b06d249cf2-partinfo-00000001-QINU I have been working with this part for several months, in which it has consistently worked. Leaky expression does appear to be a problem after 24+ hours, but it is quite stable until then.
note: the receiver construct is currently hosted on a psb2K3 plasmid.