Difference between revisions of "Part:BBa K2364000"
 
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<partinfo>BBa_K2364000 short</partinfo>
 
<partinfo>BBa_K2364000 short</partinfo>
  
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Strong mammalian expression promoter from the human cytomegalovirus.  
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  Human cytomegalovirus (CMV) immediate early enhancer and promoter. It is a generally strong mammalian expression promoter that drives constitutive expression of genes under its control. However, the strength of the promoter varies significantly between different cell types; being very strong in some (e.g. 293T and CMMT) and rather weak in others (e.g. MRC5 and MSC) (Qin Jane Yuxia, et al., 2010).
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  It has been shown in embryonic stem cells (ESCs) that the nuclear protein of 95kDa (Np95) interacts with regulatory domains of the de novo methyltransferases; Dnmt3a and Dnmt3b, and mediates silencing of the CMV promoter (Meilinger D. et al., 2009). Transcriptional silencing was also observed in rat muscles due to methylation of the CMV promoter enhancer (CMV-PE) 24h post-administration, showing decreased expression overtime (Brooks Alan R., et al., 2004) . Therefore, when using the CMV promoter, the type of cell as well as the time given to express the gene-of-interest should not be considered consistent.  
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===Usage and Biology===
 
===Usage and Biology===
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Strong mammalian expression promoter from the human cytomegalovirus.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2364000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2364000 SequenceAndFeatures</partinfo>
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Latest revision as of 22:07, 3 March 2018


pCMV 

  Human cytomegalovirus (CMV) immediate early enhancer and promoter. It is a generally strong mammalian expression promoter that drives constitutive expression of genes under its control. However, the strength of the promoter varies significantly between different cell types; being very strong in some (e.g. 293T and CMMT) and rather weak in others (e.g. MRC5 and MSC) (Qin Jane Yuxia, et al., 2010). 
  It has been shown in embryonic stem cells (ESCs) that the nuclear protein of 95kDa (Np95) interacts with regulatory domains of the de novo methyltransferases; Dnmt3a and Dnmt3b, and mediates silencing of the CMV promoter (Meilinger D. et al., 2009). Transcriptional silencing was also observed in rat muscles due to methylation of the CMV promoter enhancer (CMV-PE) 24h post-administration, showing decreased expression overtime (Brooks Alan R., et al., 2004) . Therefore, when using the CMV promoter, the type of cell as well as the time given to express the gene-of-interest should not be considered consistent.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]