Difference between revisions of "Part:BBa I737021"

 
 
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<partinfo>BBa_I737021 short</partinfo>
 
<partinfo>BBa_I737021 short</partinfo>
  
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This part constituatively expresses agarase. It is used to produce e. coli which degrade the agar substrate that they are normally grown on. Typical rate of degradation is low, it takes two to three days to degrade 4mm of agar underneath the colonies.  
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This part constituatively expresses agarase. It is used to produce e. coli which degrade the agar substrate that they are normally grown on. Typical rate of degradation is low, it takes two to three days to degrade 4mm of agar underneath the colonies with Pseudoalteromonas atlantica T6C. Degradation is visible by holding the plate at an angle in strong light, the dimples in the agar are then obvious. Currently the rate of degradation of agar by e. coli is not known because agar degradation by e. coli has not been observed yet.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:36, 26 October 2007

Constitutively expressed Agarase part.

This part constituatively expresses agarase. It is used to produce e. coli which degrade the agar substrate that they are normally grown on. Typical rate of degradation is low, it takes two to three days to degrade 4mm of agar underneath the colonies with Pseudoalteromonas atlantica T6C. Degradation is visible by holding the plate at an angle in strong light, the dimples in the agar are then obvious. Currently the rate of degradation of agar by e. coli is not known because agar degradation by e. coli has not been observed yet.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1943
    Illegal PstI site found at 366
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1943
    Illegal NheI site found at 188
    Illegal NheI site found at 983
    Illegal PstI site found at 366
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1943
    Illegal XhoI site found at 2046
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1943
    Illegal PstI site found at 366
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1943
    Illegal PstI site found at 366
    Illegal AgeI site found at 450
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1401
    Illegal SapI.rc site found at 1799
    Illegal SapI.rc site found at 1877