Difference between revisions of "Part:BBa M50083"
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<partinfo>BBa_M50083 short</partinfo> | <partinfo>BBa_M50083 short</partinfo> | ||
| − | The purpose of this plasmid is to express the human serotonin receptor (5HT1A) in yeast cells. There is a histidine tag on the extracellular side to test for correct membrane expression of the receptor in yeast cells. | + | The purpose of this plasmid is to express the human serotonin receptor (5HT1A) in yeast cells. There is a histidine tag on the extracellular side to test for correct membrane expression of the receptor in yeast cells. This is expressed under a galactose-inducible promoter, followed by a yeast Kozak sequence (pGal) for translation. It's terminated by a Cyc1 terminator. |
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Latest revision as of 23:32, 7 December 2017
Modified Yeast Serotonin Receptor
The purpose of this plasmid is to express the human serotonin receptor (5HT1A) in yeast cells. There is a histidine tag on the extracellular side to test for correct membrane expression of the receptor in yeast cells. This is expressed under a galactose-inducible promoter, followed by a yeast Kozak sequence (pGal) for translation. It's terminated by a Cyc1 terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 780
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC[1000]