Difference between revisions of "Part:BBa K2262003"
 
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__NOTOC__
 
<partinfo>BBa_K2262003 short</partinfo>
 
<partinfo>BBa_K2262003 short</partinfo>
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[[File:Composite part LA-LTP.png|800px|thumb|center|'''Figure 1.''' P<sub>T7</sub>+RBS+LA-LTP+terminator  ]]
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<br>
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<h1>'''Introduction'''</h1>
 
<h1>'''Introduction'''</h1>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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This peptide is from the LTP family, which is standing for Lipid Transfer Protein. The mechanism of LTP protein is to bind to the lipid on the cell membrane of the pathogen through electrostatic interactions and causes destabilization and permeabilization of the membrane [1]. NCTU_Formosa used it to be one of a training data for forming the antifungal peptide scoring card. 
  
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This peptide is from the LTP family, which is standing for Lipid Transfer Protein. The mechanism of LTP protein is to bind to the lipid on the cell membrane of the pathogen through electrostatic interactions and causes destabilization and permeabilization of the membrane. NCTU_Formosa used it to be one of a training data for creating our anti-fungal peptide scoring card.
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<br>
  
 
<h1>'''Experiment'''</h1>
 
<h1>'''Experiment'''</h1>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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We put T7 promoter, RBS, and LA-LTP together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+MiAMP1 is around 354 b.p. Its PCR product is around 666 b.p.
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[[File:Agarose gel LA-LTP.png|200px|thumb|center|'''Figure 2.''' P<sub>T7</sub> + RBS + LA-LTP <br>
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The DNA sequence length of P<sub>T7</sub> + RBS + LA-LTP is around 250 ~ 500 b.p. The size of its PCR product should be close to 650 ~ 700 b.p.]]
  
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We put T7 promoter, RBS, and LA-LTP together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+MiAMP1 is around 250 ~ 500 b.p.. Its PCR product is around 500 ~ 750 b.p..
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<h1>'''Safety'''</h1>
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We do the safety part which is the same as the Hv1a-lectin. See more in
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<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2262005">BBa_K2262005</a></html>.
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<h1>'''Reference'''</h1>
 
<h1>'''Reference'''</h1>
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[1] Yang X1; Li J; Li X; She R; Pei Y. "Isolation and characterization of a novel thermostable non-specific lipid transfer protein-like antimicrobial protein from motherwort (Leonurus japonicus Houtt) seeds." Peptides. 2006 Dec;27(12):3122-8. Epub 2006 Sep 18.
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Yang X1; Li J; Li X; She R; Pei Y. "Isolation and characterization of a novel thermostable non-specific lipid transfer protein-like antimicrobial protein from motherwort (Leonurus japonicus Houtt) seeds." Peptides. 2006 Dec;27(12):3122-8. Epub 2006 Sep 18.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:08, 7 December 2017


T7 Promoter+RBS+LA-LTP



Figure 1. PT7+RBS+LA-LTP+terminator



Introduction

      This peptide is from the LTP family, which is standing for Lipid Transfer Protein. The mechanism of LTP protein is to bind to the lipid on the cell membrane of the pathogen through electrostatic interactions and causes destabilization and permeabilization of the membrane [1]. NCTU_Formosa used it to be one of a training data for forming the antifungal peptide scoring card.


Experiment

      We put T7 promoter, RBS, and LA-LTP together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+MiAMP1 is around 354 b.p. Its PCR product is around 666 b.p.

Figure 2. PT7 + RBS + LA-LTP
The DNA sequence length of PT7 + RBS + LA-LTP is around 250 ~ 500 b.p. The size of its PCR product should be close to 650 ~ 700 b.p.



Reference

[1] Yang X1; Li J; Li X; She R; Pei Y. "Isolation and characterization of a novel thermostable non-specific lipid transfer protein-like antimicrobial protein from motherwort (Leonurus japonicus Houtt) seeds." Peptides. 2006 Dec;27(12):3122-8. Epub 2006 Sep 18.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]