Difference between revisions of "Part:BBa K2262003"
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<h1>'''Introduction'''</h1> | <h1>'''Introduction'''</h1> | ||
| | ||
| − | This peptide is from the LTP family, which is standing for Lipid Transfer Protein. The mechanism of LTP protein is to bind to the lipid on the cell membrane of the pathogen through electrostatic interactions and causes destabilization and permeabilization of the membrane. NCTU_Formosa used it to be one of a training data for | + | This peptide is from the LTP family, which is standing for Lipid Transfer Protein. The mechanism of LTP protein is to bind to the lipid on the cell membrane of the pathogen through electrostatic interactions and causes destabilization and permeabilization of the membrane [1]. NCTU_Formosa used it to be one of a training data for forming the antifungal peptide scoring card. |
<br> | <br> | ||
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<h1>'''Experiment'''</h1> | <h1>'''Experiment'''</h1> | ||
| | ||
| − | We put T7 promoter, RBS, and LA-LTP together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+MiAMP1 is around | + | We put T7 promoter, RBS, and LA-LTP together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+MiAMP1 is around 354 b.p. Its PCR product is around 666 b.p. |
[[File:Agarose gel LA-LTP.png|200px|thumb|center|'''Figure 2.''' P<sub>T7</sub> + RBS + LA-LTP <br> | [[File:Agarose gel LA-LTP.png|200px|thumb|center|'''Figure 2.''' P<sub>T7</sub> + RBS + LA-LTP <br> | ||
The DNA sequence length of P<sub>T7</sub> + RBS + LA-LTP is around 250 ~ 500 b.p.. The size of its PCR product should be close to 650 ~ 700 b.p..]] | The DNA sequence length of P<sub>T7</sub> + RBS + LA-LTP is around 250 ~ 500 b.p.. The size of its PCR product should be close to 650 ~ 700 b.p..]] | ||
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<h1>'''Reference'''</h1> | <h1>'''Reference'''</h1> | ||
| − | + | [1] Yang X1; Li J; Li X; She R; Pei Y. "Isolation and characterization of a novel thermostable non-specific lipid transfer protein-like antimicrobial protein from motherwort (Leonurus japonicus Houtt) seeds." Peptides. 2006 Dec;27(12):3122-8. Epub 2006 Sep 18. | |
| − | Yang X1; Li J; Li X; She R; Pei Y. "Isolation and characterization of a novel thermostable non-specific lipid transfer protein-like antimicrobial protein from motherwort (Leonurus japonicus Houtt) seeds." Peptides. 2006 Dec;27(12):3122-8. Epub 2006 Sep 18. | + | |
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Revision as of 14:34, 7 December 2017
T7 Promoter+RBS+LA-LTP
Introduction
This peptide is from the LTP family, which is standing for Lipid Transfer Protein. The mechanism of LTP protein is to bind to the lipid on the cell membrane of the pathogen through electrostatic interactions and causes destabilization and permeabilization of the membrane [1]. NCTU_Formosa used it to be one of a training data for forming the antifungal peptide scoring card.
Experiment
We put T7 promoter, RBS, and LA-LTP together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+MiAMP1 is around 354 b.p. Its PCR product is around 666 b.p.
Reference
[1] Yang X1; Li J; Li X; She R; Pei Y. "Isolation and characterization of a novel thermostable non-specific lipid transfer protein-like antimicrobial protein from motherwort (Leonurus japonicus Houtt) seeds." Peptides. 2006 Dec;27(12):3122-8. Epub 2006 Sep 18.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]