Difference between revisions of "Part:BBa J100337:Experience"
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| + | Cell type used was JM109. Data is average red fluorescence of 3 identical samples of each clone. Cells were grown in LB + AMP media. When aTc was present, its concentration was 0.2 micrograms/mL. X1-3 were all believed to be J100337 (our designed promoter) in J100205 (repClone). Each was isolated based on perceived level of red fluorescence (X1 being the most fluorescent and X3 being the least). WT was R0040 (wild type tet promoter). Negative control was J100205 alone. Positive control was J04450 (a sequence known to have a strong promoter, RBS, and RFP). J100328 was also used as a control. Table shows the ratios for each clone of red fluorescence produced in the presence of aTc divided by re fluorescence produced in the absence of aTc | ||
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Revision as of 13:36, 16 November 2017
Here is the figure for v2 of repClone AM, 2017.
Cell type used was JM109. Data is average red fluorescence of 3 identical samples of each clone. Cells were grown in LB + AMP media. When aTc was present, its concentration was 0.2 micrograms/mL. X1-3 were all believed to be J100337 (our designed promoter) in J100205 (repClone). Each was isolated based on perceived level of red fluorescence (X1 being the most fluorescent and X3 being the least). WT was R0040 (wild type tet promoter). Negative control was J100205 alone. Positive control was J04450 (a sequence known to have a strong promoter, RBS, and RFP). J100328 was also used as a control. Table shows the ratios for each clone of red fluorescence produced in the presence of aTc divided by re fluorescence produced in the absence of aTc
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