Difference between revisions of "Part:BBa K2194004"
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<partinfo>BBa_K2194004 short</partinfo> | <partinfo>BBa_K2194004 short</partinfo> | ||
− | + | This transcriptional unit codes for the genetic parts necessary to bind and transport chromate, sulfate, and related species across the cell membrane. BioBrick BBa_K2194004 is a composite part composed of negative feedback membrane stress promoter <i>PgntK</i> ([https://parts.igem.org/Part:BBa_K2194002 BBa_K2194002]), a synthetic RBS, cysPUWA proteins ([https://parts.igem.org/Part:BBa_K2194003 BBa_K2194003]), Elowitz RBS ([https://parts.igem.org/Part:BBa_B0034 BBa_B0034]), sulfate binding protein sbp ([https://parts.igem.org/Part:BBa_K2194001 BBa_K2194001]), and a synthetic terminator. | |
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+ | ===Biology and Usage=== | ||
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+ | Refer to the registry pages for <i>PgntK</i> ([https://parts.igem.org/Part:BBa_K2194002 BBa_K2194002]), cysPUWA proteins ([https://parts.igem.org/Part:BBa_K2194003 BBa_K2194003]), Elowitz RBS ([https://parts.igem.org/Part:BBa_B0034 BBa_B0034]), and sulfate binding protein sbp ([https://parts.igem.org/Part:BBa_K2194001 BBa_K2194001]) for detailed biology. | ||
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+ | Figure 1 shows the effect of cysPUWA overexpression on the cells' susceptibility to Cr(VI). The lower growth rate for cysPUWA transformants in the presence of Cr(VI) indicates a higher rate of chromium import and increased toxicity to the cell. | ||
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+ | [[File:Cys_growth.png|600px]] | ||
+ | <p class = "caption"> <b> Figure 1:</b> Effect of Chromate on <i>E. coli</i> Growth </p> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2194004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2194004 SequenceAndFeatures</partinfo> | ||
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Latest revision as of 03:57, 2 November 2017
Sulfate Transport System w/ Negative Feedback for Membrane Stress
This transcriptional unit codes for the genetic parts necessary to bind and transport chromate, sulfate, and related species across the cell membrane. BioBrick BBa_K2194004 is a composite part composed of negative feedback membrane stress promoter PgntK (BBa_K2194002), a synthetic RBS, cysPUWA proteins (BBa_K2194003), Elowitz RBS (BBa_B0034), sulfate binding protein sbp (BBa_K2194001), and a synthetic terminator.
Biology and Usage
Refer to the registry pages for PgntK (BBa_K2194002), cysPUWA proteins (BBa_K2194003), Elowitz RBS (BBa_B0034), and sulfate binding protein sbp (BBa_K2194001) for detailed biology.
Figure 1 shows the effect of cysPUWA overexpression on the cells' susceptibility to Cr(VI). The lower growth rate for cysPUWA transformants in the presence of Cr(VI) indicates a higher rate of chromium import and increased toxicity to the cell.
Figure 1: Effect of Chromate on E. coli Growth
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1480
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2296
- 1000COMPATIBLE WITH RFC[1000]