Difference between revisions of "Part:BBa K2194003"
 
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The <i>cysPUWA</i> genes encode the sulfate ABC membrane transporter in <i>Escherichia coli</i>. This system also transports related species such as chromate. BioBrick BBa_K2194003 contains the cds for <i>cysP</i>, <i>cysU</i>, <i>cysW</i>, and <i>cysA</i> in the context in which they appear in <i>E. coli</i> MG1655.
 
The <i>cysPUWA</i> genes encode the sulfate ABC membrane transporter in <i>Escherichia coli</i>. This system also transports related species such as chromate. BioBrick BBa_K2194003 contains the cds for <i>cysP</i>, <i>cysU</i>, <i>cysW</i>, and <i>cysA</i> in the context in which they appear in <i>E. coli</i> MG1655.
  
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===Biology and Usage===
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===Usage and Biology===
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The binding protein <i>cysP</i> increases the affinity for sulfate uptake; genes <i>cysU</i> and <i>cysW</i> encode the integral membrane proteins. The final cds in this BioBrick is for <i>cysA</i> which is an ATP-binding subunit.
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Figure 1 shows the effect of cysPUWA overexpression on the cells' susceptibility to Cr(VI). The lower growth rate for cysPUWA transformants in the presence of Cr(VI) indicates the higher rate of chromium import and resulting toxicity.
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[[File:Cys_growth.png|600px]]
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<p class = "caption"> <b> Figure 1:</b> Effect of Chromate on <i>E. coli</i> Growth </p>
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Latest revision as of 03:53, 2 November 2017


cysPUWA Sulfate Transport System cds

The cysPUWA genes encode the sulfate ABC membrane transporter in Escherichia coli. This system also transports related species such as chromate. BioBrick BBa_K2194003 contains the cds for cysP, cysU, cysW, and cysA in the context in which they appear in E. coli MG1655.

Biology and Usage

The binding protein cysP increases the affinity for sulfate uptake; genes cysU and cysW encode the integral membrane proteins. The final cds in this BioBrick is for cysA which is an ATP-binding subunit.

Figure 1 shows the effect of cysPUWA overexpression on the cells' susceptibility to Cr(VI). The lower growth rate for cysPUWA transformants in the presence of Cr(VI) indicates the higher rate of chromium import and resulting toxicity.


Cys growth.png

Figure 1: Effect of Chromate on E. coli Growth


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2090
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 3828