Difference between revisions of "Part:BBa K2423002"
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<partinfo>BBa_K2423002 short</partinfo> | <partinfo>BBa_K2423002 short</partinfo> | ||
| − | This part codes for a cytosolic glycosyltransferase found in Crocus sativus. The enzyme will catalyse the conversion of crocetin to α-crocin in the presence of UDP-glucose. It is a modified version of BBa_K1033112 | + | This part codes for a cytosolic glycosyltransferase found in Crocus sativus. The enzyme will catalyse the conversion of crocetin to α-crocin in the presence of UDP-glucose. It is a modified version of BBa_K1033112, with two stop codons and a SGGS linker for the His tag. BBa_K2423008 is a slightly modifed version of this part (it uses BBa_J04500 as a promoter and RBS instead). |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | Saffron, a well recognized but expensive spice, has not only uses in terms of cooking but compounds found in saffron have been shown to help with inflammation (1), neurodegenerative diseases (2) and more. Some of those compounds namely zeaxanthin, crocetin dialdehyde, crocetin and crocin are all a part of the same metabolic pathway in the plant specie ''Crocus Sativus''. Not only are these compounds in saffron helpful in terms of their potential medicinal properties, but also the fact that they are very colorful makes them interesting as organic dyes for industrial purposes. These aspects are what drew us at iGEM Uppsala 2017 to work with the pathway from zeaxanthin to crocin in the BioBrick format, but also to integrate the metabolic steps in the pathway from farnesyl pyrophospate (FPP) to zeaxanthin on the chromosome of ''Escherichia Coli''. The enzyme presented on this page, UGTCs2 catalyzes the third and final reaction in the zeaxanthin to crocin pathway. | ||
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<partinfo>BBa_K2423002 parameters</partinfo> | <partinfo>BBa_K2423002 parameters</partinfo> | ||
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| + | ===References=== | ||
| + | 1. Papandreou MA, Kanakis CD, Polissiou MG, Efthimiopoulos S, Cordopatis P, Margarity M, et al. Inhibitory Activity on Amyloid-β Aggregation and Antioxidant Properties of Crocus sativus Stigmas Extract and Its Crocin Constituents. J Agric Food Chem. 2006 Nov 1;54(23):8762–8. | ||
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| + | 2. Chen L, Qi Y, Yang X. Neuroprotective effects of crocin against oxidative stress induced by ischemia/reperfusion injury in rat retina. Ophthalmic Res. 2015;54(3):157–68. | ||
Latest revision as of 03:49, 2 November 2017
UGTCs2 under the control of a constitutive promoter (BBa_J23106)
This part codes for a cytosolic glycosyltransferase found in Crocus sativus. The enzyme will catalyse the conversion of crocetin to α-crocin in the presence of UDP-glucose. It is a modified version of BBa_K1033112, with two stop codons and a SGGS linker for the His tag. BBa_K2423008 is a slightly modifed version of this part (it uses BBa_J04500 as a promoter and RBS instead).
Usage and Biology
Saffron, a well recognized but expensive spice, has not only uses in terms of cooking but compounds found in saffron have been shown to help with inflammation (1), neurodegenerative diseases (2) and more. Some of those compounds namely zeaxanthin, crocetin dialdehyde, crocetin and crocin are all a part of the same metabolic pathway in the plant specie Crocus Sativus. Not only are these compounds in saffron helpful in terms of their potential medicinal properties, but also the fact that they are very colorful makes them interesting as organic dyes for industrial purposes. These aspects are what drew us at iGEM Uppsala 2017 to work with the pathway from zeaxanthin to crocin in the BioBrick format, but also to integrate the metabolic steps in the pathway from farnesyl pyrophospate (FPP) to zeaxanthin on the chromosome of Escherichia Coli. The enzyme presented on this page, UGTCs2 catalyzes the third and final reaction in the zeaxanthin to crocin pathway.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 593
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 766
Illegal AgeI site found at 1121 - 1000COMPATIBLE WITH RFC[1000]
References
1. Papandreou MA, Kanakis CD, Polissiou MG, Efthimiopoulos S, Cordopatis P, Margarity M, et al. Inhibitory Activity on Amyloid-β Aggregation and Antioxidant Properties of Crocus sativus Stigmas Extract and Its Crocin Constituents. J Agric Food Chem. 2006 Nov 1;54(23):8762–8.
2. Chen L, Qi Y, Yang X. Neuroprotective effects of crocin against oxidative stress induced by ischemia/reperfusion injury in rat retina. Ophthalmic Res. 2015;54(3):157–68.