Difference between revisions of "Part:BBa K2340000:Design"
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===Source=== | ===Source=== | ||
− | + | The sequence of this biobrick is synthesized by Integrated DNA Technologies (IDT). | |
− | + | The p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A gene sequence was a gift from Jennifer Doudna (Addgene plasmid # 83485<sup>1</sup>). Three nucleotides were edited to remove the illegal restriction enzyme cutting sites. To ensure efficient protein expression a codon usage table was used<sup>2</sup>. | |
+ | The sequence of the GFP gene was obtained from the biobrick library (BBa_K648013). | ||
===References=== | ===References=== | ||
+ | <sup>1</sup><b>Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection</b>. East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian R, Doudna JA.<i> Nature. 2016 Sep 26. doi: 10.1038/nature19802.</i> 10.1038/nature19802 PubMed 27669025 | ||
+ | |||
+ | <sup>2</sup>GenScript (2017). GenScript Codon Usage Frequence Table Tool. [WWW document]. URL https://www.genscript.com/tools/codon-frequency-table |
Latest revision as of 03:48, 2 November 2017
dCAS13a linked to GFP with NLS
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1112
Illegal BglII site found at 2048
Illegal BglII site found at 2312
Illegal BglII site found at 2816
Illegal BglII site found at 3302
Illegal BglII site found at 3410
Illegal BglII site found at 3740 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 662
Illegal SapI.rc site found at 3421
Design Notes
Addition of LINK and NLS sequence
Source
The sequence of this biobrick is synthesized by Integrated DNA Technologies (IDT). The p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A gene sequence was a gift from Jennifer Doudna (Addgene plasmid # 834851). Three nucleotides were edited to remove the illegal restriction enzyme cutting sites. To ensure efficient protein expression a codon usage table was used2. The sequence of the GFP gene was obtained from the biobrick library (BBa_K648013).
References
1Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian R, Doudna JA. Nature. 2016 Sep 26. doi: 10.1038/nature19802. 10.1038/nature19802 PubMed 27669025
2GenScript (2017). GenScript Codon Usage Frequence Table Tool. [WWW document]. URL https://www.genscript.com/tools/codon-frequency-table