Difference between revisions of "Part:BBa K2267044"
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<partinfo>BBa_K2267044 short</partinfo> | <partinfo>BBa_K2267044 short</partinfo> | ||
− | + | This composite part is based on the Lux quorum-sensing system from Vibrio fischerii. It consists of a Lux receiver device and a GFP reporter that is activated in the presence of a 3O-C6 AHL signal. | |
+ | |||
+ | <!-- Add more about the biology of this part here> | ||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | <!-- --> | ||
+ | Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression. | ||
+ | In this case, the constitutively expressed LuxR transcriptional regulator ( https://parts.igem.org/Part:BBa_K2267032 we improved) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device. | ||
+ | |||
+ | https://parts.igem.org/Part:BBa_K2267040 is Control, | ||
+ | |||
+ | part1: https://parts.igem.org/Part:BBa_K2267043 | ||
+ | |||
+ | part3: https://parts.igem.org/Part:BBa_K2267041 | ||
+ | |||
+ | part4: https://parts.igem.org/Part:BBa_K2267042 | ||
+ | |||
+ | part6: https://parts.igem.org/Part:BBa_K2267044 | ||
+ | |||
+ | part8: https://parts.igem.org/Part:BBa_K2267045 | ||
+ | |||
+ | ===result=== | ||
+ | we did point mutation on https://parts.igem.org/Part:BBa_R0062 and the exact spots are | ||
+ | |||
+ | plux1: https://parts.igem.org/Part:BBa_K2267029 | ||
+ | |||
+ | plux3: https://parts.igem.org/Part:BBa_K2267030 | ||
+ | |||
+ | plux4: https://parts.igem.org/Part:BBa_K2267031 | ||
+ | |||
+ | plux6: https://parts.igem.org/Part:BBa_K2267032 | ||
+ | |||
+ | plux8: https://parts.igem.org/Part:BBa_K2267046 | ||
+ | |||
+ | listed below. | ||
+ | We linked gfp reporter genes | ||
+ | luxr-plux1-gfp: https://parts.igem.org/Part:BBa_K2267043 | ||
+ | |||
+ | luxr-plux3-gfp: https://parts.igem.org/Part:BBa_K2267041 | ||
+ | |||
+ | luxr-plux4-gfp: https://parts.igem.org/Part:BBa_K2267042 | ||
+ | |||
+ | luxr-plux6-gfp: https://parts.igem.org/Part:BBa_K2267044 | ||
+ | |||
+ | luxr-plux8-gfp: https://parts.igem.org/Part:BBa_K2267045 | ||
+ | |||
+ | on them respectively to test the function of modified promoters. | ||
+ | https://static.igem.org/mediawiki/2017/e/e6/T--TUST_China--part_part_results.png | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Latest revision as of 03:38, 2 November 2017
LuxR-I-Plux-GFP-6
This composite part is based on the Lux quorum-sensing system from Vibrio fischerii. It consists of a Lux receiver device and a GFP reporter that is activated in the presence of a 3O-C6 AHL signal.
Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression. In this case, the constitutively expressed LuxR transcriptional regulator ( https://parts.igem.org/Part:BBa_K2267032 we improved) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device.
https://parts.igem.org/Part:BBa_K2267040 is Control,
part1: https://parts.igem.org/Part:BBa_K2267043
part3: https://parts.igem.org/Part:BBa_K2267041
part4: https://parts.igem.org/Part:BBa_K2267042
part6: https://parts.igem.org/Part:BBa_K2267044
part8: https://parts.igem.org/Part:BBa_K2267045
result
we did point mutation on https://parts.igem.org/Part:BBa_R0062 and the exact spots are
plux1: https://parts.igem.org/Part:BBa_K2267029
plux3: https://parts.igem.org/Part:BBa_K2267030
plux4: https://parts.igem.org/Part:BBa_K2267031
plux6: https://parts.igem.org/Part:BBa_K2267032
plux8: https://parts.igem.org/Part:BBa_K2267046
listed below. We linked gfp reporter genes luxr-plux1-gfp: https://parts.igem.org/Part:BBa_K2267043
luxr-plux3-gfp: https://parts.igem.org/Part:BBa_K2267041
luxr-plux4-gfp: https://parts.igem.org/Part:BBa_K2267042
luxr-plux6-gfp: https://parts.igem.org/Part:BBa_K2267044
luxr-plux8-gfp: https://parts.igem.org/Part:BBa_K2267045
on them respectively to test the function of modified promoters.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 837
Illegal NheI site found at 860 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 928
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1452
Illegal BsaI.rc site found at 2179