Difference between revisions of "Part:BBa K2364021"
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<partinfo>BBa_K2364021 short</partinfo> | <partinfo>BBa_K2364021 short</partinfo> | ||
| − | . | + | DsRed expressed under the control of the CMV constitutive promoter. A bacterial constitutive promoter expressing an sfGFP is used for aid in the cloning process. |
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<partinfo>BBa_K2364021 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2364021 SequenceAndFeatures</partinfo> | ||
| + | Transfection experiment of the construct together with a transfection marker in the Caco-2 cell line. We observe DsRed fluorescence and a limited transfection efficiency, possibly due to low transfection rates of Caco-2 cells. | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2364021 parameters</partinfo> | <partinfo>BBa_K2364021 parameters</partinfo> | ||
| − | + | https://static.igem.org/mediawiki/2017/9/9e/Greekom_secondPart1.png | |
Latest revision as of 03:35, 2 November 2017
pCMV-DsRed-J23100-sfGFP-SV40 polyA
DsRed expressed under the control of the CMV constitutive promoter. A bacterial constitutive promoter expressing an sfGFP is used for aid in the cloning process.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1320
Illegal NheI site found at 1343 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 585
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1528
- 1000COMPATIBLE WITH RFC[1000]
Transfection experiment of the construct together with a transfection marker in the Caco-2 cell line. We observe DsRed fluorescence and a limited transfection efficiency, possibly due to low transfection rates of Caco-2 cells.
Functional Parameters
