Difference between revisions of "Part:BBa K2309028:Experience"
 
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<h2>1. Gel electrophoresis</h2>
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We use gel electrophoresis to show the result of construction.
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https://static.igem.org/mediawiki/2017/4/43/Gel_electrophoresis.jpeg">
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            <figure>
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                <img width="650px" src="https://static.igem.org/mediawiki/2017/4/43/Gel_electrophoresis.jpeg">
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From left to right: pSB1C3+LL-37 (cut by PstI, uncut); pSB1C3+GF-17 (cut by pstI, uncut); 1kb DNA maker; pEt28a (+)+3x AMPs (uncut, cut)
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                <figcaption>
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                    <strong>Fig.1 From left to right: pSB1C3+LL-37 (cut by PstI, uncut); pSB1C3+GF-17 (cut by pstI, uncut); 1kb DNA maker;
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                        pEt28a (+)+3x AMPs (uncut, cut)</strong>
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Latest revision as of 03:35, 2 November 2017


We use gel electrophoresis to show the result of construction.

Gel_electrophoresis.jpeg">

From left to right: pSB1C3+LL-37 (cut by PstI, uncut); pSB1C3+GF-17 (cut by pstI, uncut); 1kb DNA maker; pEt28a (+)+3x AMPs (uncut, cut)


The inhibitio ring is used for test the production of peptide to S. aureus

3P.png

the results of MIC for mixed peptide as follows:

Test11.png

for the biofilm formation assay, the result was shown as follows:

Abs_123xjtlu.png

Applications of BBa_K2309028

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