Difference between revisions of "Part:BBa K2260000:Design"
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| − | The phaCBA operon was codon optimized for use in <i>E. coli</i> using | + | The phaCBA operon was codon optimized for use in <i>E. coli</i> using this <a href="http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=9606">codon optimization table</a>. Four restrictions sites present in the original operon obtained from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a> were removed, allowing our part to be compatible in all iGEM RFC standards. New restriction sites introduced from codon optimization were also removed. Lastly, histidine tags were added to the N-termini of each protein in the operon for protein purification. |
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Latest revision as of 03:33, 2 November 2017
PhaCBA operon w/ Histidine tags
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The phaCBA operon was codon optimized for use in E. coli using this codon optimization table. Four restrictions sites present in the original operon obtained from BBa_K1149051 were removed, allowing our part to be compatible in all iGEM RFC standards. New restriction sites introduced from codon optimization were also removed. Lastly, histidine tags were added to the N-termini of each protein in the operon for protein purification.
Source
The naturally existing phaCAB operon is present in R. eutropha H16. The operon sequence was taken from BBa_K1149051 and rearranged.