Difference between revisions of "Part:BBa K2260000:Design"

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===Design Notes===
 
===Design Notes===
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The phaCBA operon was codon optimized for use in <i>E. coli</i>  using the codon optimization table. Furthermore, four restrictions sites present in the original operon obtained from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a> were removed, including any new restriction sites introduced from codon optimization.
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The phaCBA operon was codon optimized for use in <i>E. coli</i>  using this <a href="http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=9606">codon optimization table</a>. Four restrictions sites present in the original operon obtained from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a> were removed, allowing our part to be compatible in all iGEM RFC standards. New restriction sites introduced from codon optimization were also removed. Lastly, histidine tags were added to the N-termini of each protein in the operon for protein purification.
 
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===Source===
 
===Source===
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Insert bacterial species' genome(s) here.
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The naturally existing phaCAB operon is present in <i>R. eutropha</i> H16. The operon sequence was taken from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a>  and rearranged.</p>
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===References===
 
===References===

Latest revision as of 03:33, 2 November 2017


PhaCBA operon w/ Histidine tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The phaCBA operon was codon optimized for use in E. coli using this codon optimization table. Four restrictions sites present in the original operon obtained from BBa_K1149051 were removed, allowing our part to be compatible in all iGEM RFC standards. New restriction sites introduced from codon optimization were also removed. Lastly, histidine tags were added to the N-termini of each protein in the operon for protein purification.

Source

The naturally existing phaCAB operon is present in R. eutropha H16. The operon sequence was taken from BBa_K1149051 and rearranged.

References