Difference between revisions of "Part:BBa K2433004:Design"
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===Design Notes===
 
===Design Notes===
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The VirB1 promoter was designed by taking the promoter region of virB1 from the pTiC58 - a Ti plasmid from Agrobacterium Tumefaciens. The nucleotide at position 339 was changed to a T on the coding strand to eliminate an XbaI cutsite.  
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The <i>virB1</i> promoter was designed by taking the promoter region of <i>virB1</i> from the pTiC58 - a Ti plasmid from <i>Agrobacterium tumefaciens</i>. The nucleotide at position 339 was changed to a T to eliminate an XbaI cutsite.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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NEED TO ADD
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AE007871.2

Latest revision as of 03:30, 2 November 2017


VirB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 118


Design Notes

The virB1 promoter was designed by taking the promoter region of virB1 from the pTiC58 - a Ti plasmid from Agrobacterium tumefaciens. The nucleotide at position 339 was changed to a T to eliminate an XbaI cutsite.

Source

Upstream regulatory region of virB1 from pTiC58.

References

AE007871.2