Difference between revisions of "Part:BBa K2381019"

 
 
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<partinfo>BBa_K2381019 short</partinfo>
 
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the light-activated split dCas9, which fused the rational split dCas9 with light-induced dimerization protein, can activated under the irritation of blue light(470nm)
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The light-activated split Luciferase, used to verify the function of light-induced dimerization protein.
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this part can response to blue light (470nm), rapidly bind to the corresponding parts, BBa_K2381013, due to eletrostatic interaction and dimerize. Its cofactor for light sensing is flavin adenine dinucleotide (FAD). After dimerization the split luciferase will complement to recover its abillity to generate fluorescenece.
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<img src="https://static.igem.org/mediawiki/parts/d/df/HZAU_2017_MagLuc.png" width="600px"/>
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<b>Fig.1 </b>Prove the function of pMag and nMag. n=1
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:29, 2 November 2017


light-activated split Luciferase

The light-activated split Luciferase, used to verify the function of light-induced dimerization protein. this part can response to blue light (470nm), rapidly bind to the corresponding parts, BBa_K2381013, due to eletrostatic interaction and dimerize. Its cofactor for light sensing is flavin adenine dinucleotide (FAD). After dimerization the split luciferase will complement to recover its abillity to generate fluorescenece.


Fig.1 Prove the function of pMag and nMag. n=1



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2595
    Illegal AgeI site found at 1442
    Illegal AgeI site found at 2734
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2236