Difference between revisions of "Part:BBa K2364025"
 
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<partinfo>BBa_K2364025 short</partinfo>
 
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DsRed expressed by a constitutive promoter CMV with binding targets for miR-21 fused in the 3' untranslated region.
  
 
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<partinfo>BBa_K2364025 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2364025 SequenceAndFeatures</partinfo>
  
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Transfection experiments using pCMV-DsRed-T21-SV40 polyA and a transfection marker in two cancer cell lines, Caco-2 (first picture) and A549 (second picture). miR-21 is upregulated in these cancer cell lines. As a result, we observe a brillian inhibition effect, and no DsRed fluorescence is detected.
 
   
 
   
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2364025 parameters</partinfo>
 
<partinfo>BBa_K2364025 parameters</partinfo>
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[[File:IC16_STAR1stgraph.png|700px|center|]]
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[[File:Greekom pandorra thirdImage.png]]
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https://static.igem.org/mediawiki/2017/0/0d/Greekom_prwtoPart1.png
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Latest revision as of 03:29, 2 November 2017


pCMV-DsRed-T21-SV40 polyA

DsRed expressed by a constitutive promoter CMV with binding targets for miR-21 fused in the 3' untranslated region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 585
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Transfection experiments using pCMV-DsRed-T21-SV40 polyA and a transfection marker in two cancer cell lines, Caco-2 (first picture) and A549 (second picture). miR-21 is upregulated in these cancer cell lines. As a result, we observe a brillian inhibition effect, and no DsRed fluorescence is detected.

Functional Parameters


Greekom_prwtoPart1.png Greekom_prwtoopart2.png