Difference between revisions of "Part:BBa K2364025"
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<partinfo>BBa_K2364025 short</partinfo> | <partinfo>BBa_K2364025 short</partinfo> | ||
| − | . | + | DsRed expressed by a constitutive promoter CMV with binding targets for miR-21 fused in the 3' untranslated region. |
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<partinfo>BBa_K2364025 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2364025 SequenceAndFeatures</partinfo> | ||
| − | + | Transfection experiments using pCMV-DsRed-T21-SV40 polyA and a transfection marker in two cancer cell lines, Caco-2 (first picture) and A549 (second picture). miR-21 is upregulated in these cancer cell lines. As a result, we observe a brillian inhibition effect, and no DsRed fluorescence is detected. | |
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2364025 parameters</partinfo> | <partinfo>BBa_K2364025 parameters</partinfo> | ||
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| − | + | https://static.igem.org/mediawiki/2017/0/0d/Greekom_prwtoPart1.png | |
| + | https://static.igem.org/mediawiki/2017/4/42/Greekom_prwtoopart2.png | ||
Latest revision as of 03:29, 2 November 2017
pCMV-DsRed-T21-SV40 polyA
DsRed expressed by a constitutive promoter CMV with binding targets for miR-21 fused in the 3' untranslated region.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 585
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Transfection experiments using pCMV-DsRed-T21-SV40 polyA and a transfection marker in two cancer cell lines, Caco-2 (first picture) and A549 (second picture). miR-21 is upregulated in these cancer cell lines. As a result, we observe a brillian inhibition effect, and no DsRed fluorescence is detected.
Functional Parameters
