Difference between revisions of "Part:BBa K2364027"

 
 
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<partinfo>BBa_K2364027 short</partinfo>
 
<partinfo>BBa_K2364027 short</partinfo>
  
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DsRed expressed under the constitutive promoter CMV. Binding sites for the miR-145-5p, miR-143-3p and the synthetic miRNA FF4 are fused in the 3'-untranslated region.  
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2364027 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2364027 SequenceAndFeatures</partinfo>
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DsRed expression is regulated by 2 endogenous miRNAs (miR-145, miR143) and 1 synthetic one (miR-FF4). 4 repeats of the miR-372-3p and miR-373-3p binding sites are fused into the 3'-untranslated region and 3 repeats of the miR-FF4 are found downstream of the aforementioned 2 binding sites.
  
  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2364027 parameters</partinfo>
 
<partinfo>BBa_K2364027 parameters</partinfo>
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Latest revision as of 03:14, 2 November 2017


pCMV-DsRed-T145-T143-TFF4-SV40 polyA

DsRed expressed under the constitutive promoter CMV. Binding sites for the miR-145-5p, miR-143-3p and the synthetic miRNA FF4 are fused in the 3'-untranslated region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 585
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

DsRed expression is regulated by 2 endogenous miRNAs (miR-145, miR143) and 1 synthetic one (miR-FF4). 4 repeats of the miR-372-3p and miR-373-3p binding sites are fused into the 3'-untranslated region and 3 repeats of the miR-FF4 are found downstream of the aforementioned 2 binding sites.


Functional Parameters