Difference between revisions of "Part:BBa K2346003"

Latest revision as of 03:12, 2 November 2017

Nitrate/nitrite responsive promoter with amplifier

This is a composite part and a functional improved device for Part:BBa_K216005(https://parts.igem.org/Part:BBa_K216005). The Pyear promoter will be switched on under the presence of nitrate or nitrite, translating P2 ogr. The P2 ogr will cause inducible PF promoter to produce even stronger downstream PoPS. We attached a RBS for convince of registry users. We ligated this part with a GFP+terminator. Conclusion As we can see from the graph, there is significant difference between NiSAP’s response to nitrate(40mM) and Pyear+GFP’s response to nitrate(40mM). After 3 hours in the plate reader, NiSAP’s A.U. is nearly twice the magnitude of Pyear+GFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 508
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2346003 short</partinfo>
-Nitrate/nitrite responsive promoter with amplifier
+This is a composite part and a functional improved device for Part:BBa_K216005(https://parts.igem.org/Part:BBa_K216005). The Pyear promoter will be switched on under the presence of nitrate or nitrite, translating P2 ogr. The P2 ogr will cause inducible PF promoter to produce even stronger downstream PoPS. We attached a RBS for convince of registry users.
+We ligated this part with a GFP+terminator.
+[[Image:BBa K2346003.png]]
+Conclusion
+As we can see from the graph, there is significant difference between NiSAP’s response to nitrate(40mM) and Pyear+GFP’s response to nitrate(40mM). After 3 hours in the plate reader, NiSAP’s A.U. is nearly twice the magnitude of Pyear+GFP.
 <!-- Add more about the biology of this part here