Difference between revisions of "Part:BBa K2346006"

Latest revision as of 03:10, 2 November 2017

Enhanced Sensor for Nitrite/Nitrate

This is a functional improved device for BBa_K381001(https://parts.igem.org/Part:BBa_K381001). We replaced the original reporter gene GFP with brighter reporter gene sfGFP, in the hope to improve the sensitivity for the device to detect nitrite and nitrate. The Pyear promoter will be switched on under the presence of nitrate or nitrite, translating P2 ogr. The P2 ogr will cause inducible PF promoter to produce even stronger downstream PoPS. We attached a RBS for convince of registry users.

As we can see from the graph, there is significant difference between NiSAP’s response to nitrate(40mM) and Pyear+GFP’s response to nitrate(40mM). After 3 hours in the plate reader, NiSAP’s A.U. is nearly twice the magnitude of Pyear+GFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 141

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 <partinfo>BBa_K2346006 short</partinfo>
-This is a functional improved device for BBa_K381001(https://parts.igem.org/Part:BBa_K381001). We replaced the original reporter gene GFP with brighter reporter gene sfGFP,  in the hope to improve the sensitivity for the device to detect nitrite and nitrate.
+This is a functional improved device for BBa_K381001(https://parts.igem.org/Part:BBa_K381001). We replaced the original reporter gene GFP with brighter reporter gene sfGFP,  in the hope to improve the sensitivity for the device to detect nitrite and nitrate. The Pyear promoter will be switched on under the presence of nitrate or nitrite, translating P2 ogr. The P2 ogr will cause inducible PF promoter to produce even stronger downstream PoPS. We attached a RBS for convince of registry users.
+[[Image:BBa K2346006.png]]
+As we can see from the graph, there is significant difference between NiSAP’s response to nitrate(40mM) and Pyear+GFP’s response to nitrate(40mM). After 3 hours in the plate reader, NiSAP’s A.U. is nearly twice the magnitude of Pyear+GFP.
 <!-- Add more about the biology of this part here