Difference between revisions of "Part:BBa K1039000"
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The attP site is the phage attachment site for the phiC31 phage. The site is recognized by phiC31 integrase. The phiC31 integrase catalyzes a unidirectional strand exchange between a 39 bp attP site and a 34 bp attB site (Groth et al., 2000). The enzyme works by making a synapse between attP and attB, making double strand breaks producing a 2 bp sticky overhang, exchanging the strands, and re-ligating them in the recombinant configuration (attL and attR). | The attP site is the phage attachment site for the phiC31 phage. The site is recognized by phiC31 integrase. The phiC31 integrase catalyzes a unidirectional strand exchange between a 39 bp attP site and a 34 bp attB site (Groth et al., 2000). The enzyme works by making a synapse between attP and attB, making double strand breaks producing a 2 bp sticky overhang, exchanging the strands, and re-ligating them in the recombinant configuration (attL and attR). | ||
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| + | Reference: Amy C. Groth, Michele P. Calos, Phage Integrases: Biology and Applications, Journal of Molecular Biology, Volume 335, Issue 3, 16 January 2004, Pages 667-678, ISSN 0022-2836, http://dx.doi.org/10.1016/j.jmb.2003.09.082. Sequence and Features | ||
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| + | =Improvement and Characterization by Fudan_China 2017= | ||
| + | *'''Group:''' Fudan_China 2017 | ||
| + | *'''Author:''' Haiyun Liu, Fudan University | ||
| + | We improved this part and characterized the kinetic characterization and the orthogonality between the integrase and some other serine recombinase using this improved attP site ([[Part:BBa_K24600011|BBa_K2460011]]). For more information, go to [[Part:BBa_K1039012|phiC31 integrase]]. | ||
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Latest revision as of 03:07, 2 November 2017
attP site for phiC31 integrase
The attP site is the phage attachment site for the phiC31 phage. The site is recognized by phiC31 integrase. The phiC31 integrase catalyzes a unidirectional strand exchange between a 39 bp attP site and a 34 bp attB site (Groth et al., 2000). The enzyme works by making a synapse between attP and attB, making double strand breaks producing a 2 bp sticky overhang, exchanging the strands, and re-ligating them in the recombinant configuration (attL and attR).
Reference: Amy C. Groth, Michele P. Calos, Phage Integrases: Biology and Applications, Journal of Molecular Biology, Volume 335, Issue 3, 16 January 2004, Pages 667-678, ISSN 0022-2836, http://dx.doi.org/10.1016/j.jmb.2003.09.082. Sequence and Features
Improvement and Characterization by Fudan_China 2017
- Group: Fudan_China 2017
- Author: Haiyun Liu, Fudan University
We improved this part and characterized the kinetic characterization and the orthogonality between the integrase and some other serine recombinase using this improved attP site (BBa_K2460011). For more information, go to phiC31 integrase.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]