Difference between revisions of "Part:BBa K2448025:Design"
 
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===Design Notes===
 
===Design Notes===
  
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This biosensor was built using the Universal Biosensing Chassis ([[Part:BBa_K2448023|BBa_K2448023]], [[Part:BBa_K2448024|BBa_K2448024]]). It based on the PsiR transcription factor from ''Agrobacterium tumefaciens'' ([[Part:BBa_K2448006|BBa_K2448006]]) and its associated promoter pPsiA ([[Part:BBa_K2448010|BBa_K2448010]]).
+
This biosensor was built using the Universal Biosensing Chassis ([[Part:BBa_K2448023|BBa_K2448023]], [[Part:BBa_K2448024|BBa_K2448024]]). It is based on the PsiR transcription factor from ''Agrobacterium tumefaciens'' ([[Part:BBa_K2448006|BBa_K2448006]]) and its associated promoter pPsiA ([[Part:BBa_K2448010|BBa_K2448010]]).
  
 
===Source===
 
===Source===
 +
 +
DNA synthesis of individual parts and assembly by Golden Gate.
  
 
===References===
 
===References===

Latest revision as of 02:59, 2 November 2017


Psicose Biosensor pPsiA-PsiR from Agrobacterium tumefaciens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1337
    Illegal XhoI site found at 62
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 104
    Illegal AgeI site found at 791
    Illegal AgeI site found at 2351
    Illegal AgeI site found at 2463
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biosensor was built using the Universal Biosensing Chassis (BBa_K2448023, BBa_K2448024). It is based on the PsiR transcription factor from Agrobacterium tumefaciens (BBa_K2448006) and its associated promoter pPsiA (BBa_K2448010).

Source

DNA synthesis of individual parts and assembly by Golden Gate.

References