Difference between revisions of "Part:BBa K2460010"
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<partinfo>BBa_K2460010 short</partinfo> | <partinfo>BBa_K2460010 short</partinfo> | ||
| − | + | This part is improved from [[Part:BBa_K1039035|BBa_K1039035]]. | |
| + | This is a recombination site attB for phiC31 integrase ([[Part:BBa_K1039012|BBa_K1039012]]). When used with phiC31 attP ([[Part:BBa_K2460006|BBa_K2460006]]) and phiC31 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site. | ||
| + | For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to [[Part:BBa_K1039012|phiC31 integrase]]. | ||
| + | |||
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Revision as of 02:53, 2 November 2017
φC31 attB
This part is improved from BBa_K1039035. This is a recombination site attB for phiC31 integrase (BBa_K1039012). When used with phiC31 attP (BBa_K2460006) and phiC31 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site. For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to phiC31 integrase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]