Difference between revisions of "Part:BBa K2486014"

 
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<partinfo>BBa_K2486014 short</partinfo>
 
<partinfo>BBa_K2486014 short</partinfo>
  
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A modified version of BBa_K2486010
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This module works as a reporter for lactate an tetracycline presence. The circuit's key part is the PdtxR promoter ([https://parts.igem.org/Part:BBa_K2486175 BBa_K2486175]),a synthetic promoter based on [https://parts.igem.org/Part:BBa_J23108 BBa_J23108] promoter from [https://parts.igem.org/Promoters/Catalog/Anderson Anderson Promoter] Collection  with a DtxR binding site carefully added to it. We adopted dtxR operator in our promoter instead of fur operator because of dtxR different operator sequence, wich reduces its interaction with endogenous sites for this regulator. While the fur Fur regulator is derived form <i>E. coli</i> the DtxR is derived from <i>Corynebacterium diphtheriae</i>
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# BBa_B0034: a strong RBS from [the https://parts.igem.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Community_Collection Community Collection]
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# BBa_K2486024: a 6 base pair spacer for optimal expression.
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# BBa_K082003: a GFP with LVA tag
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===Characterization===
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https://static.igem.org/mediawiki/2017/b/bd/T--USP-Brazil--Detecttion_Iron_Lactate_concept_%287%29.PNG
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:50, 2 November 2017


PdtxR reporter circuit (GFP)

This module works as a reporter for lactate an tetracycline presence. The circuit's key part is the PdtxR promoter (BBa_K2486175),a synthetic promoter based on BBa_J23108 promoter from Anderson Promoter Collection with a DtxR binding site carefully added to it. We adopted dtxR operator in our promoter instead of fur operator because of dtxR different operator sequence, wich reduces its interaction with endogenous sites for this regulator. While the fur Fur regulator is derived form E. coli the DtxR is derived from Corynebacterium diphtheriae

  1. BBa_B0034: a strong RBS from [the https://parts.igem.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Community_Collection Community Collection]
  2. BBa_K2486024: a 6 base pair spacer for optimal expression.
  3. BBa_K082003: a GFP with LVA tag

Characterization

T--USP-Brazil--Detecttion_Iron_Lactate_concept_%287%29.PNG

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716