Difference between revisions of "Part:BBa K2381010"

 
 
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__NOTOC__
 
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<partinfo>BBa_K2381010 short</partinfo>
 
<partinfo>BBa_K2381010 short</partinfo>
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<p>In this part, the light-induced dimerization protein,pMag[https://parts.igem.org/Part:BBa_K2381000], is fused with the C end of split dCas9 through rigid linker.<br/>
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the linker is changed from flexibility to rigid amino acid
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The alpha-helix structure of the rigid linker renders more cooperativity between split dCas9 and dimerization protein.<br/>
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Its amion acid sequence is A(EAAAK)3A, in which the flanking Ala guarantees a flexibility conjunction, which can decrease the effect on the function of protein. </p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:36, 2 November 2017


Cod1+ rigid linker+pMagFast

In this part, the light-induced dimerization protein,pMag[1], is fused with the C end of split dCas9 through rigid linker.
The alpha-helix structure of the rigid linker renders more cooperativity between split dCas9 and dimerization protein.
Its amion acid sequence is A(EAAAK)3A, in which the flanking Ala guarantees a flexibility conjunction, which can decrease the effect on the function of protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2277
  • 1000
    COMPATIBLE WITH RFC[1000]