Difference between revisions of "Part:BBa K2398029"

Revision as of 02:36, 2 November 2017

OrganosiliconeRS-geneIII see: https://parts.igem.org/wiki/index.php?title=Part:BBa_K2398555 We present the organosilicon-sensing riboswitch that regulates phage propagation by enhancing organosilicon-depending expression of geneIII that is vital for host cell infection.

Sequence and Features

Assembly Compatibility:

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INCOMPATIBLE WITH RFC[10]
Unknown
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INCOMPATIBLE WITH RFC[12]
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INCOMPATIBLE WITH RFC[21]
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INCOMPATIBLE WITH RFC[23]
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COMPATIBLE WITH RFC[25]
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COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K2398029 short</partinfo>
-see: https://parts.igem.org/wiki/index.php?title=Part:BBa_K2398000
+see: https://parts.igem.org/wiki/index.php?title=Part:BBa_K2398555
+We present the organosilicon-sensing riboswitch that regulates phage propagation by enhancing organosilicon-depending expression of geneIII that is vital for host cell infection.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+The upstream riboswitch sequence can be adapted to any organosilicon of interest using the {{#tag:html|<a href="http://2017.igem.org/Team:Heidelberg/Software/MAWS">MAWS</a>}} 2.0 software. So far, variants of this part have been used only for proof-of-principle applications but this part is designed to have a variety of applications in the cytochrome c engineering, for example, the phage-related discontinuous evolution ({{#tag:html|<a href="http://2017.igem.org/Team:Heidelberg/Predcel">PREDCEL</a>}}) approach.
+===Characterisation of the related part====
+The results of the proof-of-concept of the related part are depicted in Fig. 1-3.
+[[File:T--Heidelberg--Compounds2.png|thumb|center|Figure 1: Used compounds for the characterization of the organosilicon-sensing riboswitch]]
+[[File:T--Heidelberg--RawDataAptamer.png|thumb|center|Figure 2: Light emission detection of the NanoLuc reaction for different riboswitch activators and concentrations. Addition of compound (5) to the reaction resulted in increased enzyme activity as indicated by the two bars on the left-hand side compared to the compound (1) reaction.]]
+[[File:T-Heidelberg--FoldActivityProductEduct.png|thumb|center|Figure 3: Fold enzyme activity upon addition of compound (5) compared to compound (1) at either 5 mM concentration (left bar) or 15 mM concentration (right bar). At both concentrations, compound (5) exceeded the reaction activity of compound (1) by 1.5 fold.]]
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 <span class='h3bb'>Sequence and Features</span>