Difference between revisions of "Part:BBa K2348011"
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<partinfo>BBa_K2348011 short</partinfo><br>
 
<partinfo>BBa_K2348011 short</partinfo><br>
 
This part actually is a composite part containing the asr promoter [https://parts.igem.org/Part:BBa_K2348001 BBa_K2348001] and mCardinal [https://parts.igem.org/Part:BBa_K2348002 BBa_K2348002]. Additionally, between promoter and cardinal an additional RBS, a TEV-site and a f-degron are inserted. Also, mCardinal is 6xHis-taged and the part ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.<br>
 
This part actually is a composite part containing the asr promoter [https://parts.igem.org/Part:BBa_K2348001 BBa_K2348001] and mCardinal [https://parts.igem.org/Part:BBa_K2348002 BBa_K2348002]. Additionally, between promoter and cardinal an additional RBS, a TEV-site and a f-degron are inserted. Also, mCardinal is 6xHis-taged and the part ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.<br>
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TEV-site and f-degron allow a fast and controllable degradation of proteins and in combination with the fast maturation times of our fluorescence proteins, allow a fast colour change. This enables shorter time spans between signals when controlling the robot’s movement. Due to a change of our reactor design this feature is no longer needed because the pH is only changed in a small amount of media which is discarded after measurement.
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TEV-site and F-degron allow a fast and controllable degradation of mNeonGreen wich, in combination with the fast maturation time of mNeonGreen, lead to a fast colour change when used together with our other pH-sensitive part [https://parts.igem.org/Part:BBa_K2348012 BBa_K2348012]
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The 6xHis-tag allows detection of mNeonGreen on protein level and the T7-terminator stops translation. The part itself was created using extension PCR and overlap PCR.  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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The part is used as pH regulated mCardinal expression device. Data of expression is shown in the asr Promoter part [https://parts.igem.org/Part:BBa_K2348001 BBa_K2348001].<br><br>
 
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<span class='h3bb'><b>Sequence and Features</b></span><br>
 
<span class='h3bb'><b>Sequence and Features</b></span><br>
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https://static.igem.org/mediawiki/2017/thumb/9/96/Asr_construct_Graz.jpeg/800px-Asr_construct_Graz.jpeg
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https://static.igem.org/mediawiki/2017/thumb/9/96/Asr_construct_Graz.jpeg/800px-Asr_construct_Graz.jpeg <br>
 
<partinfo>BBa_K2348011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2348011 SequenceAndFeatures</partinfo>
  

Latest revision as of 02:31, 2 November 2017


asr promoter + mCardinal
This part actually is a composite part containing the asr promoter BBa_K2348001 and mCardinal BBa_K2348002. Additionally, between promoter and cardinal an additional RBS, a TEV-site and a f-degron are inserted. Also, mCardinal is 6xHis-taged and the part ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.
TEV-site and F-degron allow a fast and controllable degradation of mNeonGreen wich, in combination with the fast maturation time of mNeonGreen, lead to a fast colour change when used together with our other pH-sensitive part BBa_K2348012

Usage and Biology

The part is used as pH regulated mCardinal expression device. Data of expression is shown in the asr Promoter part BBa_K2348001.

Sequence and Features
800px-Asr_construct_Graz.jpeg


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 639
    Illegal BsaI.rc site found at 828