Difference between revisions of "Part:BBa K2348011"
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| − | <partinfo>BBa_K2348011 short</partinfo> | + | <partinfo>BBa_K2348011 short</partinfo><br> |
| + | This part actually is a composite part containing the asr promoter [https://parts.igem.org/Part:BBa_K2348001 BBa_K2348001] and mCardinal [https://parts.igem.org/Part:BBa_K2348002 BBa_K2348002]. Additionally, between promoter and cardinal an additional RBS, a TEV-site and a f-degron are inserted. Also, mCardinal is 6xHis-taged and the part ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.<br> | ||
| + | TEV-site and F-degron allow a fast and controllable degradation of mNeonGreen wich, in combination with the fast maturation time of mNeonGreen, lead to a fast colour change when used together with our other pH-sensitive part [https://parts.igem.org/Part:BBa_K2348012 BBa_K2348012] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | The part is used as pH regulated mCardinal expression device. Data of expression is shown in the asr Promoter part [https://parts.igem.org/Part:BBa_K2348001 BBa_K2348001].<br><br> | |
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| − | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><b>Sequence and Features</b></span><br> |
| + | https://static.igem.org/mediawiki/2017/thumb/9/96/Asr_construct_Graz.jpeg/800px-Asr_construct_Graz.jpeg <br> | ||
<partinfo>BBa_K2348011 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2348011 SequenceAndFeatures</partinfo> | ||
Latest revision as of 02:31, 2 November 2017
asr promoter + mCardinal
This part actually is a composite part containing the asr promoter BBa_K2348001 and mCardinal BBa_K2348002. Additionally, between promoter and cardinal an additional RBS, a TEV-site and a f-degron are inserted. Also, mCardinal is 6xHis-taged and the part ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.
TEV-site and F-degron allow a fast and controllable degradation of mNeonGreen wich, in combination with the fast maturation time of mNeonGreen, lead to a fast colour change when used together with our other pH-sensitive part BBa_K2348012
Usage and Biology
The part is used as pH regulated mCardinal expression device. Data of expression is shown in the asr Promoter part BBa_K2348001.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 639
Illegal BsaI.rc site found at 828