Difference between revisions of "Part:BBa K2460002"
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<partinfo>BBa_K2460002 short</partinfo> | <partinfo>BBa_K2460002 short</partinfo> | ||
| − | This is a recombination site attB for phiBT1. | + | This is a recombination site attB for phiBT1 integrase ([[Part:BBa_K2460001|BBa_K2460001]]). When used with phiBT1 attP ([[Part:BBa_K2460003|BBa_K2460003]]) and phiBT1 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site. |
| + | For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to [[Part:BBa_K2460001|phiBT1 integrase]]. | ||
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Latest revision as of 02:31, 2 November 2017
φBT1 attB
This is a recombination site attB for phiBT1 integrase (BBa_K2460001). When used with phiBT1 attP (BBa_K2460003) and phiBT1 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site. For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to phiBT1 integrase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]