Difference between revisions of "Part:BBa K2403002"
 
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You can transport high molecular mRNA into cytoplasm if you can use this parts BBa_2403000.
 
You can transport high molecular mRNA into cytoplasm if you can use this parts BBa_2403000.
 
Rev protein binds to RRE(Rev Response Element).
 
Rev protein binds to RRE(Rev Response Element).
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<!-- Add more about the biology of this part here-->
 
<!-- Add more about the biology of this part here-->
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This part contains the RRE (Rev Response Element) of HIV-1 along with a T7 promoter for RNA expression. Together with the HIV-1 Rev protein part (BBa_K2403000), it can be used to efficiently export target RNAs from the eukaryotic nucleus out into the cytoplasm. Details are summarized in '''[https://parts.igem.org/Part:BBa_K2403000  this link]'''.
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This part contains the RRE (Rev Response Element) of HIV-1 along with a T7 promoter for RNA expression. Together with the HIV-1 Rev protein part ('''[https://parts.igem.org/Part:BBa_K2403000 BBa_K2403000]'''), it can be used to efficiently export target RNAs from the eukaryotic nucleus out into the cytoplasm. Details are summarized in '''[https://parts.igem.org/Part:BBa_K2403000  this link]'''.
  
 
===Reference===
 
===Reference===
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[1]https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041468/pdf/gku304.pdf
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[1] Ichiro Taniguchi, Naoto Mabuchi and Mutsuhito Ohno (2014) HIV-1 Rev protein specifies the viral RNA export
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pathway by suppressing TAP/NXF1 recruitment <i>Nucleic Acids Research</i>, 6645–665
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Latest revision as of 02:31, 2 November 2017


T7 promoter>RRE(Rev Response Element)

You can transport high molecular mRNA into cytoplasm if you can use this parts BBa_2403000. Rev protein binds to RRE(Rev Response Element).


This part contains the RRE (Rev Response Element) of HIV-1 along with a T7 promoter for RNA expression. Together with the HIV-1 Rev protein part (BBa_K2403000), it can be used to efficiently export target RNAs from the eukaryotic nucleus out into the cytoplasm. Details are summarized in this link.

Reference

[1] Ichiro Taniguchi, Naoto Mabuchi and Mutsuhito Ohno (2014) HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment Nucleic Acids Research, 6645–665


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]