Difference between revisions of "Part:BBa K2403002"
| (5 intermediate revisions by 2 users not shown) | |||
| Line 6: | Line 6: | ||
Rev protein binds to RRE(Rev Response Element). | Rev protein binds to RRE(Rev Response Element). | ||
| + | |||
| + | <!-- Add more about the biology of this part here--> | ||
| + | This part contains the RRE (Rev Response Element) of HIV-1 along with a T7 promoter for RNA expression. Together with the HIV-1 Rev protein part ('''[https://parts.igem.org/Part:BBa_K2403000 BBa_K2403000]'''), it can be used to efficiently export target RNAs from the eukaryotic nucleus out into the cytoplasm. Details are summarized in '''[https://parts.igem.org/Part:BBa_K2403000 this link]'''. | ||
| + | |||
| + | ===Reference=== | ||
| + | [1] Ichiro Taniguchi, Naoto Mabuchi and Mutsuhito Ohno (2014) HIV-1 Rev protein specifies the viral RNA export | ||
| + | pathway by suppressing TAP/NXF1 recruitment <i>Nucleic Acids Research</i>, 6645–665 | ||
| − | |||
| − | |||
<!-- --> | <!-- --> | ||
Latest revision as of 02:31, 2 November 2017
T7 promoter>RRE(Rev Response Element)
You can transport high molecular mRNA into cytoplasm if you can use this parts BBa_2403000. Rev protein binds to RRE(Rev Response Element).
This part contains the RRE (Rev Response Element) of HIV-1 along with a T7 promoter for RNA expression. Together with the HIV-1 Rev protein part (BBa_K2403000), it can be used to efficiently export target RNAs from the eukaryotic nucleus out into the cytoplasm. Details are summarized in this link.
Reference
[1] Ichiro Taniguchi, Naoto Mabuchi and Mutsuhito Ohno (2014) HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment Nucleic Acids Research, 6645–665
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]